The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The measurement can be finished within 1.0 hour, with no need for 4-5 hours. The detection range is from 20 to 15000 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.

Sensitivity

The minimum detectable dose (MOD) of human IFN-γ is typically 8 pg/ml.

Specificity

This assay recognizes natural and recombinant human IFN- γ.

Simple ProceduresSimple Standard PreparationAccurate Standard CurveKit Contents & Storage Conditions

Part	Part #		Storage of Opened/Reconstituted
Human IFN-γ Microplate	TBS3230A	96-well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ.	Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IFN-γ Standard	TBS3230B	100 µL of Recombinant human IFN- γ protein (145.8ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3230C	2.2 ml of HRP-Human IFN- γ antibody.	May be stored for up to 3 months at 2-8℃
Assay Diluent	TBS3230D	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 ml of concentrated solution (10x).
TMB Substrate	TBS3000T	12 ml of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates. The kit contains sufficient materials to run an ELISA on one 96-well plate.

Related Product

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-4 ELISA (TBS3221)
4. Human IL-6 ELISA (TBS3223)
5. Human IL-7 ELISA (TBS3224)

Additional information

SIZE	1 x 96-well plate

TBS3230_human_IFN_gamma_elisa_V07_2021-REVISED

Ao, Z., Wu, Z., Cai, H., Hu, L., Li, X., Kaurich, C., Chang, J., Gu, M., Cheng, L., Lu, X., & Guo, F. (2022). Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(22), e2201478. https://doi.org/10.1002/advs.202201478

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TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.