Boron is an essential micronutrient in plants and is involved in maintaining robust cell walls, cell membranes, and reproductive tissues. Although boron is common in the soil in its natural state as a borate mineral, the amount of boron available to plants is quite small. As a result, boron deficiency is the second most common micronutrient deficiency among crop plants. In order to keep plant boron levels in a healthy range, supplementation to the soil via fertilizers and additives is often required. If not regulated, a lack or excess of boron may significantly lower crop yield. In the biotech industry, sodium borohydride is commonly used to conjugate antibodies and typically needs to be removed from the final product, especially for therapeutic antibodies. Tribioscience’s Boron Colorimetric Assay provides a convenient and reliable means to measure boron. In the assay, borate complexes with azomethine-H to create a colored compound that can be measured at 420nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.

Applications:

Direct Assays: Boron in water, plant tissues, soil samples, and antibody conjugation solutions.

Key Features:

Fast: Just one step that takes less than 10 minutes. Accurate: Linear detection range of 0.1-20 μg/mL (0.1-20 ppm) boron in 50μL samples. Simple and High-Throughput: The reagents are ready to use. The kit can be used as a robust method for a high-throughput 96-well or 384-well plate.

Kit Contents (for 200 Assays):

Assay Buffer A: 12mL	Boron Standard: 200μL (100 μg/mL)
Assay Buffer B: 12mL

Storage and Handling:

Store kit at -20℃. Shelf life of six months.

Example Standard Curve

Additional information

SIZE (kit)	200 Assays

TBS2301_Boron_Colorimetric_Assay

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TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.