Tribioscience’s Botulinum Neurotoxin Type A (BoNT-A) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type A (BoNT-A) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 46.9 to 3000 pg/mL. The levels of Botulinum Neurotoxin Type A (BoNT-A) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type A (BoNT-A) protein.

Typical Data

This standard curve (R²=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of BoNT-A is typically 46 pg/mL.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant BoNT-A.

No cross-reactivity with others.

Simple Procedures (Fig. 1)

Simple Standard Preparation 

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#		Storage of Opened/Reconstituted
Botulinum Neurotoxin Type A Microplate	TBS31007A	96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-A.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Botulinum Neurotoxin Type A Standard	TBS31007B	50 µl of Recombinant Botulinum Neurotoxin Type A (30 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS31007C	2.1 mL of biotin-Botulinum Neurotoxin Type A antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS31007D	200 µL of streptavidin HRP.
Assay Diluent	TBS31007E	25 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates. The kit contains sufficient materials to run an ELISA on one 96-well plate.

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Additional information

SIZE	1 x 96-well plate

TBS31007 Botulinum Neurotoxin Type A (BoNT-A) Elisa datasheet 6-2023 (3)

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TribioScience 是一家私人控股的生物技术公司，于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验，我们还作为 CRO（研究合同组织）为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming.  We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.