DOTAP/DC-CHOL Cationic Liposomes

Product code: F50101V

Lipid composition: DOTAP/DC-Cholesterol (50:50 mol/mol)

Mean particle size: 50-80 nm (by DSL)

Lipid concentration: 40 mM (24.7 mg/mL)

Volume: 2.0mL

Bulk solution: D5W (dextrose in milli-Q water)

Shelf-life: 6 months guaranteed for sterility for unopened vials stored at 2-8 degree C (guaranteed starting from the date of delivery, replacement only)

DOTAP: 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt)

DC-CHOL: DC-Cholesterol∙HCl, 3b-[N-(N’,N’-dimethylaminoethane)-caramoyl] chelesterol hydrochloride

Attachment of class B CpG ODN onto DOTAP/DC-chol liposome in nasal vaccine formulations augments antigen-specific immune responses in mice.

Tada R, Muto S, Iwata T, Hidaka A, Kiyono H, Kunisawa J, Aramaki Y.BMC Res Notes. 2017 Jan 26;10(1):68. doi: 10.1186/s13104-017-2380-8.PMID: 28126014

Nasal vaccination with pneumococcal surface protein A in combination with cationic liposomes consisting of DOTAP and DC-chol confers antigen-mediated protective immunity against Streptococcus pneumoniae infections in mice.

Tada R, Suzuki H, Takahashi S, Negishi Y, Kiyono H, Kunisawa J, Aramaki Y.Int Immunopharmacol. 2018 Aug;61:385-393.

Comparison of the depot effect and immunogenicity of liposomes based on dimethyldioctadecylammonium (DDA), 3β-[N-(N’,N’-Dimethylaminoethane)carbomyl] cholesterol (DC-Chol), and 1,2-Dioleoyl-3-trimethylammonium propane (DOTAP): prolonged liposome retention mediates stronger Th1 responses.

Henriksen-Lacey M, Christensen D, Bramwell VW, Lindenstrøm T, Agger EM, Andersen P, Perrie Y.Mol Pharm. 2011 Feb 7;8(1):153-61.

Additional information