Avian Influenza Virus (AIV) TaqMan Probe RT-PCR Detection Kit is one step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the simultaneous detection of AIV H5, H7 and H9 subtypes in avian samples in one reaction tube. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting targets.

AIV TaqMan Probe RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer probes, internal control, positive control, negative control, and buffer. The AIV target genes H5, H7, and H9 are labeled with fluorescence Fam, and internal control is labeled with Hex.

This kit is used for the detection of AIV H5, H7, and H9 subtypes in avian samples.

• One-step complete qRT-PCR in a single tube.
• Reduce contamination in the operating process.
• Accurate and simultaneous detection of AIV H5, H7, and H9 subtypes.
• 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.

1. TBS6025: Microbial DNA Magnetic Extraction
2. TBS42018: Trichothecene-producing Fusarium Species TaqProbe qPCR Detection
3. TBS42019: Fusarium Species qPCR Detection
4. TBS42020: Universal Aspergillus qPCR
5. TBS42021: Aspergillus Flavus qPCR

Additional information

TBS42039_AIV_h5-h7-h9_Taqman_qRT-PCR_V01_2023