CV-HLVD-LCV Multiplex RT-PCR Detection Kit is used for the detection of cryptic virus (CV), hop latent viroid(HLVD), and lettuce chlorosis (LCV) from the cannabis plant in one step RT-PCR reaction. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy-to-use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting CV, HLVd, and LCV from plant samples.

Tribioscience Multiplex RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer probes, internal control, positive control, negative control, and buffer. The 3 target genes are labeled with a specific fluorescence color: Cy5 for Cryptic virus, Fam for HLVD, ROX for lettuce chlorosis virus, and Hex for internal control.

• This kit is used for the detection of CV, HLVd, and LCV infection in plant samples.

• One-step complete qRT-PCR in a single tube.
• Reduce contamination in the operating process.
• Accurate detection and quantification of HLVd target gene.
• 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.

Store all components at -20°C in a non-frost-free freezer. Shelf life is 12 months after receipt. The kit is shipped on ice.

1. TBS6025: Microbial DNA Magnetic Extraction
2. TBS42018: Trichothecene-producing Fusarium Species TaqProbe qPCR Detection
3. TBS42015: HLVD RT-qPCR detection
4. TBS42019: Fusarium Species qPCR Detection
5. TBS42020: Universal Aspergillus qPCR

Additional information

TBS42016_Taqman_qRT-PCR_CV-HLVd-LCV_V01_2023