The Aspergillus niger qPCR Kit has been designed to identify A. niger species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns. The Aspergillus niger qPCR Kit includes Aspergillus niger target positive and negative controls, PCR internal controls labeled with Hex, a qPCR super mix, and the primer-probe mix in which the probe has been labeled with FAM for Aspergillus niger. These aid in a straightforward interpretation of the results.

Applications:

Detect Aspergillus niger DNA in food and animal feed.

Key Features:

• High sensitivity and specificity for A. niger detection.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: Just add DNA Template and water.
• No cross reactivity with other species.

Kit Contents:

Name	100x rxn	200x rxn
qPCR Super mix (N1)	0.8mL	1.6mL
Primer-Probe Mix (N2)	0.6mL	1.2mL
Positive Control DNA (N+)	60μL	100μL
Negative Control DNA (N–)	60μL	100μL

The A. niger probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

Storage Conditions:

The kit is shipped on ice. All components should be stored at -20°C in a frost-free freezer. Shelf life is 12 months after receiving.

Additional information

SIZE	200 Reactions, 100 Reactions

Data Sheet:

TBS42023_Aspergillus-Niger_detection

MSDS:

TBS42023_ASP_NIGER_TAQ_MSDS_2022

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