Many countries in the world regulate the cultivation and trade with genetically modified organisms (GMO). The enforcement of such regulations depends on the ability to detect and quantify the presence of GMOs in food, feed, and seed products. The real-time qPCR method is sensitive and robust and is regarded as the gold standard for GMO analysis.

The Maize GMO MON87411 qPCR Kit is designed for identifying GMO marker MON87411 presence or absence in food, feed, and seed products using real-time quantitative polymerase chain reaction(qPCR), primers, and labeled probe. The Kit includes maize GMO MON87411 Positive and Negative controls, PCR internal controls, qPCR Super Mix, maize GMO MON87411 Prime-Probe Mix, in which the probe is labeled with Fam, and Hex is labeled for PCR internal control. These aids in the straightforward interpretation of the results.

KEY FEATURES

• Highly sensitivity and specificity for identification of GMO MON87411.
• High efficiency: the optimal systemic conditions for PCR amplification.
• Streamlined protocol: Just add DNA Template and water.
• No cross reactivity with others.

PCR PROTOCOL

1. DNA extraction: The methods for DNA extraction can be used for any suitable preparation of DNA purification from food samples. We recommend that the Fast genomic DNA extraction method be used for this purpose (catalog: TBS6008).

2. Set up a PCR reaction for each sample in 20 µL

Reaction Component	Volume (µL)
qPCR Super Mix	7.0
Primer-probe Mix	5.0
Nuclease-free Water	3.0
DNA sample	5.0
Final Volume	20 µL

Internal control should be included as below: Positive Control or Negative control (5 µL /reaction).

3. Suggested PCR conditions

Step	Amplification	PCR
	HOLD	CYCLE (40 cycles)
		Denature	Anneal/ Extend
Temperature	95 °C	95 °C	60 °C
Time	1 min	15 sec	60 sec

RELATIVE PRODUCTS

1. TBS6008: Fast Genomic DNA Extraction
2. TBS6025: Microbial DNA Magnetic Extraction
3. TBS43001: Maize GMO 98140 qPCR Detection
4. TBS43003: Maize GMO DBT418 qPCR Detection
5. TBS43004: Maize GMO LY038 qPCR Detection

Additional information

SIZE	100 Reactions

TBS43006_Maize_GMO MON87411_qPCR kit_V01_2021

1 review for Maize GMO Mon87411 qPCR Detection

1. 5 out of 5

   jchong – August 31, 2022

   Saw no issues with the kit, the protocol was clear and simple allowing for quick RT-qPCR analysis. The kit was also effective in amplifying any detection of maize GMO.

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